Journal: International Journal of Molecular Sciences

Article Title: Hepatoprotective Effect of Allium ochotense Extracts on Chronic Alcohol-Induced Fatty Liver and Hepatic Inflammation in C57BL/6 Mice

doi: 10.3390/ijms25063496

Figure Lengend Snippet: List of the antibody information used in this study.

Article Snippet: FAS , CSB-PA06354A0Rb , 1:1000 , Cusabio (Wuhan, China).

Techniques: Concentration Assay

Journal: Cell Death and Differentiation

Article Title: Characterizing the regulatory Fas (CD95) epitope critical for agonist antibody targeting and CAR-T bystander function in ovarian cancer

doi: 10.1038/s41418-023-01229-7

Figure Lengend Snippet: Details of commercial kits, antibodies, and other reagents.

Article Snippet: Anti-Fas (Human) , Cell Signaling Technology , Cat #4233.

Techniques: Virus, Recombinant, Plasmid Preparation, Proliferation Assay, Modification, Protease Inhibitor, Transfection, Generated, Software, Imaging, Chromatography

Journal: Cancer Control : Journal of the Moffitt Cancer Center

Article Title: Systemic Inflammatory Biomarkers Predict Survival of Patients Treated With Tyrosine Kinase Inhibitors for Metastatic Renal Cell Carcinoma

doi: 10.1177/10732748231197511

Figure Lengend Snippet: Representative image of infiltrating CD8 + T cells and CD4 + T cells. Representative image of infiltrating CD8 + T cells in the high (A) and low (B) NLR groups. CD4 + T cells in the high (C) and low (D) NLR groups. CD8 + T cells in the high (E) and low (F) MLR groups. CD4 + T cells in the high (G) and low (H) MLR groups.

Article Snippet: The next day, the sections were incubated with the secondary antibody, rabbit anti-human CD4 (ab133616, Abcam; concentration 1:200) at 37°C for 20 minutes.

Techniques:

Journal: Cancer Control : Journal of the Moffitt Cancer Center

Article Title: Systemic Inflammatory Biomarkers Predict Survival of Patients Treated With Tyrosine Kinase Inhibitors for Metastatic Renal Cell Carcinoma

doi: 10.1177/10732748231197511

Figure Lengend Snippet: Association between the NLR and T cell infiltration in mRCC. CD8 + T cell (A) and CD4 + T cell (B) density in the low and high NLR groups.

Article Snippet: The next day, the sections were incubated with the secondary antibody, rabbit anti-human CD4 (ab133616, Abcam; concentration 1:200) at 37°C for 20 minutes.

Techniques:

Journal: Cellular oncology (Dordrecht)

Article Title: Fatty acid synthase as a new therapeutic target for HER2-positive gastric cancer.

doi: 10.1007/s13402-023-00769-x

Figure Lengend Snippet: Fig. 3 FASN expression levels in HER2+ GC cell lines and patients and GTR0455- implanted animals according to anti-HER2 agent treatment. a-d, FASN expression evaluated by qRT‒PCR and Western blotting in OE19 (a), N87 (b), OE33 (c) and GTR0455 (d) cells treated with L or DMSO for 48 h or 72 h. Blots were probed with anti-FASN or actin (load- ing control) antibodies. Actin was used to normalize protein loading. Autoradiographs were acquired at different exposure times to obtain optimal image resolution. e and f, FASN expression evaluated by qRT‒ PCR (e) and Western blotting (f) in GTR0455 xenografts. NOD-SCID mice (n = 6/group) implanted with T-resistant GTR0455 cells were treated with T (30 mg/kg) or vehicle. Box plots, means ± SEMs. Significance was calculated by a two-tailed unpaired t test. Blots were probed with anti-FASN or actin (loading control) antibod- ies. Autoradiographs were acquired at the same exposure times. g, Box plots of H-score values of FASN IHC pre- and post-T therapy (n = 5); signifi- cance was calculated by a two- tailed paired t test. h, Images of FASN IHC in two pairs of pre- (on the left) and posttherapy (on the right) FFPE tumor samples, showing a diffuse increase in cytoplasmic immunoreactivity (20 × magnification)

Article Snippet: The immunohistochemical analysis was performed on formalin-fixed paraffin-embedded (FFPE) 2 μm tumor sections, subjected to antigen retrieval using EDTA buffer (pH 8) and then immunostained with a primary monoclonal antibody anti-human FASN (1:50; clone C20G5, #3180S, Cell Signaling Technology).

Techniques: Expressing, Western Blot, Control, Two Tailed Test

Journal: Cellular oncology (Dordrecht)

Article Title: Fatty acid synthase as a new therapeutic target for HER2-positive gastric cancer.

doi: 10.1007/s13402-023-00769-x

Figure Lengend Snippet: Fig. 5 Anti-FASN treatment decreases HER2 activation in HER2+ GC cell lines. a-d. Western blotting analyses of OE19 (a), N87 (b), OE33 (c) and GTR0455 (d) protein extracts from cells treated with DMSO or TVB3166 at different concentrations (5 mM, 10 mM and 20 mM) for 72 h. Blots were probed with anti-HER2 and anti-pHER2 antibodies. Actin was used to normalize protein loading. Autoradio- graphs were acquired at differ- ent exposure times to obtain optimal image resolution

Article Snippet: The immunohistochemical analysis was performed on formalin-fixed paraffin-embedded (FFPE) 2 μm tumor sections, subjected to antigen retrieval using EDTA buffer (pH 8) and then immunostained with a primary monoclonal antibody anti-human FASN (1:50; clone C20G5, #3180S, Cell Signaling Technology).

Techniques: Activation Assay, Western Blot

Journal: Bioscience Reports

Article Title: Muramyl dipeptide CD10 monoclonal antibody immunoconjugates inhibited acute leukemia in nude mice

doi: 10.1042/BSR20222668

Figure Lengend Snippet: Immunohistochemistry staining of CD4 ( A ) and CD8 ( B ) T-cell regions in nude mice from different groups with tumors. The results were shown as the percentage of tumor area (%) (staining area/ tumor area), the percentage of CD4-positive cells ( C ) in different groups and the percentage of CD8-positive cells ( D ) in different groups. * P <0.05 vs. PBS ( n =6).

Article Snippet: Subsequently, paraffin sections were incubated overnight at 4°C with the rabbit antibodies against human CD4 (Abcam, ab133616, Cambridge, U.K., 1:250), human CD8 (Abcam, ab4055, Cambridge, U.K., 1:200), human CD19 (Abcam, ab134114, Cambridge, U.K., 1:20), mouse CD14 (Abcam, ab182032, Cambridge, U.K., 1:1000) and mouse CD56 (Abcam, ab220360, Cambridge, U.K., 1:2000).

Techniques: Immunohistochemistry, Staining